Mechanistic Analysis of Ion Association between Dendrigraft Poly-l-lysine and 8-Anilino-1-naphthalenesulfonate at Liquid|Liquid Interfaces
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چکیده
منابع مشابه
Effect of Dendrigraft Generation on the Interaction between Anionic Polyelectrolytes and Dendrigraft Poly(l-Lysine)
In this present work, three generations of dendrigraft poly(L-Lysine) (DGL) were studied regarding their ability to interact with linear poly (acrylamide-co-2-acrylamido-2-methyl-1propanesulfonate) (PAMAMPS) of different chemical charge densities (30% and 100%). Frontal analysis continuous capillary electrophoresis (FACCE) was successfully applied to determine binding constants and binding stoi...
متن کاملDetermination of dendrigraft poly-L-lysine diffusion coefficients by taylor dispersion analysis.
This work focuses on the physicochemical characterization of dendrigraft poly-L-lysines (DGLs) obtained by polymerization of N-carboxyanhydride in buffered water (pH 6.5). Diffusion coefficients (D) and hydrodynamic radii (Rh) of five successive DGL generations were determined by Taylor dispersion analysis (TDA). To our knowledge, this is the first experimental work using TDA for the characteri...
متن کاملTryptophan and 8-anilino-1-naphthalenesulfonate compete for binding to trp repressor.
Anilinonaphthalenesulfonate (ANS) and tryptophan compete for binding to the trp repressor protein; thus, the fluorescence decrease associated with ANS dissociation can be used as a fluorometric marker for tryptophan binding to the protein. Using this approach, the tryptophan equilibrium dissociation constant was measured at 25 degrees C to be 3.7 (+/- 1.2) X 10(-5) M, a value which compares fav...
متن کاملAn improved protocol for better detection of protein using 8-anilino-1-naphthalenesulfonate.
This study describes the optimum conditions at the staining time and the signal intensity for using 8-anilino-1-naphthalenesulfonate (ANS) as a fluorescent probe to detect proteins in SDS-PAGE. Using the optimized protocol, protein can be easily detected by short time fixing (20 min) and washing (2 × 5 min), followed by 10 min of staining. As low as 1-2 ng of the protein band can be detected, a...
متن کاملBiodegradable nanoparticles composed of dendrigraft poly-L-lysine for gene delivery.
We developed novel gene vectors composed of dendrigraft poly-L-lysine (DGL). The transgene expression efficiency of the pDNA/DGL complexes (DGL complexes) was markedly higher than that of the control pDNA/poly-L-lysine complex. However, the DGL complexes caused cytotoxicity and erythrocyte agglutination at high doses. Therefore, γ-polyglutamic acid (γ-PGA), which is a biodegradable anionic poly...
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ژورنال
عنوان ژورنال: Langmuir
سال: 2018
ISSN: 0743-7463,1520-5827
DOI: 10.1021/acs.langmuir.8b00131